IVF Vitrification Media - The Kitazato Method

Kitazato vitrification media is widely considered best-in-class for the cryopreservation of oocytes and embryos, achieving superior clinical outcomes and consistently high survival rates. The Cryotop® Method, developed by Kitazato, is implemented in over 2,500,000 clinical cases annually across more than 115 countries and 3,000 assisted reproduction centres, with its excellence certified by hundreds of scientific publications. This widespread adoption and strong scientific backing underscore its proven performance in leading IVF clinics globally. The method boasts unparalleled survival rates for human specimens at every developmental stage, including 94-96% for oocytes, 97-98% for embryos, and 97-100% for PGT embryos and blastocysts. These high rates directly contribute to the successful birth of hundreds of thousands of healthy babies, demonstrating its efficacy and reliability.

A key advantage of Kitazato's vitrification system lies in its simple, safe, versatile, and easy-to-apply nature, along with its scientifically formulated media composition. The entirely synthetic media incorporates crucial components such as Hydroxypropyl cellulose (HPC) to prevent contamination risks and reduce mechanical stress, Trehalose for enhanced cellular membrane protection, and a combination of Dimethyl sulfoxide (DMSO) and ethylene glycol to ensure optimal outcomes with reduced toxicity. Additionally, gentamicin is included to prolong the media's shelf life. The media works effectively at room temperature, allowing for convenient and safe exchange between water and cryoprotectants while preserving cell membrane integrity. Kitazato further enhances consistency and laboratory efficiency by offering the same products for both oocyte and embryo vitrification. Combined with the Cryotop® device's design, which facilitates exceptionally rapid cooling and warming rates (e.g., -23,000 °C/minute cooling and 42,000 °C/minute warming), these features are paramount for achieving high survival rates. Kitazato's investment in extensive training programmes worldwide also ensures that embryologists are well-equipped to master the technique, further solidifying its reputation for consistent and high-quality results.